Skip to main content
Immunology logoLink to Immunology
. 1971 Oct;21(4):649–657.

Immunogenicity and specificity of collagen

XII. Demonstration by immunofluorescence and haemagglutination of antibodies with different specificity to human collagen

C Steffen, M Dichtl, W Knapp, H Brunner
PMCID: PMC1408234  PMID: 4941375

Abstract

A comparative investigation, using haemagglutination, haemagglutination-inhibition and immunofluorescence, was performed for demonstration of antibodies with different types of collagen specificity. In rabbit antisera to acid-soluble human collagen a fraction of species-specific antibodies to human collagen and a fraction of general, non-species-specific collagen antibodies could be demonstrated in good accordance by both techniques. Calf, rabbit, rat and guinea-pig collagen were used as heterologous control preparations.

Full text

PDF
649

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Davison P. F., Levine L., Drake M. P., Rubin A., Bump S. The serologic specificity of tropocollagen telopeptides. J Exp Med. 1967 Aug 1;126(2):331–346. doi: 10.1084/jem.126.2.331. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. ROTHBARD S., WATSON R. F. Antigenicity of rat collagen. Demonstration of antibody to rat collagen in the renal glomeruli of rats by fluorescence microscopy. J Exp Med. 1961 Jun 1;113:1041–1051. doi: 10.1084/jem.113.6.1041. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Rothbard S., Watson R. F. Demonstration by immunofluorescence of the fixation of perfused antibody to human collagen in human kidney. J Exp Med. 1967 Apr 1;125(4):595–606. doi: 10.1084/jem.125.4.595. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Rothbard S., Watson R. F. Immunologic relations among various animal collagens. J Exp Med. 1965 Sep 1;122(3):441–454. doi: 10.1084/jem.122.3.441. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. STEFFEN C., TIMPL R. [Studies on the significance of collagen in rheumatism serology]. Z Rheumaforsch. 1962 Dec;21:417–441. [PubMed] [Google Scholar]
  6. Steffen C., Carmann H., Schuster F., Tausch G., Bösch J., Freilinger G. Untersuchungen über die Autoantikörpereigenschaft von Kollagenantikörpern und ihre Vorkommen in der Synovia von Patienten mit rheumatoider Arthritis. Z Rheumaforsch. 1971 Mar-Apr;30(3):92–97. [PubMed] [Google Scholar]
  7. Steffen C., Dichtl M., Brunner H. Immunogenicity and specificity of collagen. XI. Determination of three different determinants on human collagen. Z Immunitatsforsch Allerg Klin Immunol. 1970 Oct;140(4):408–416. [PubMed] [Google Scholar]
  8. Steffen C., Schuster F., Tausch G., Timpl R., Pecker I. Weitere Untersuchungen über Kollagenantikörper bei Patienten mit primär chronischer Polyarthritis. Klin Wochenschr. 1968 Sep 15;46(18):976–981. doi: 10.1007/BF01745585. [DOI] [PubMed] [Google Scholar]
  9. Steffen C., Timpl R. Immunogenicity and specificity of collagen. X. Investigations on collagen determinants by inhibition experiments with peptides obtained after multiple degradation. Z Immunitatsforsch Allerg Klin Immunol. 1970 Jun;139(5):455–467. [PubMed] [Google Scholar]
  10. Steffen C., Timpl R., Wolff I. Immunogenicity and specificity of collagen. V. Demonstration of three different antigenic determinants on calf collagen. Immunology. 1968 Jul;15(1):135–144. [PMC free article] [PubMed] [Google Scholar]
  11. Steffen C., Timpl R., Wolff I. Immunogenität und Spezifität von Kollagen. 3. Erzeugung und Nachweis von Anti-Kollagen-Antikörpern und serologischer Vergleich von nativem und denaturiertem löslichem Kollagen. Z Immunitatsforsch Allerg Klin Immunol. 1967 Nov;134(1):91–107. [PubMed] [Google Scholar]
  12. The T. H., Feltkamp T. E. Conjugation of fluorescein isothiocyanate to antibodies. II. A reproducible method. Immunology. 1970 Jun;18(6):875–881. [PMC free article] [PubMed] [Google Scholar]
  13. Timpl R., Wolff I., Furthmayr H., Steffen C. Immunogenicity and specificity of collagen. VI. Separation of antibody fractions with restricted specificity from anti-collagen sera using an immunoadsorbent technique. Immunology. 1968 Jul;15(1):145–151. [PMC free article] [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES