Figure 5.

Effect of magnetic field on transfection of PCR products. (A) The 293T cells were transfected with PCR-DNA/polyMAG or PCR-DNA/SPIONs in presence of a static magnetic field for 20 min, and medium replaced after 4 h, or cells were routinely transfected with either PEI, lipofectamine or calcium phosphate. (B) The 293T cells and (C) synoviocytes were transfected with PCR-DNA/polyMAG and placed either on a static magnetic plate for 5 min, or a pulsating magnetic field was applied for 5 min, or the pulsating field was applied after the static field. Control cells were not exposed to any magnetic field. Numbers 1 and 2 represent the sequence of application of the magnetic fields. Results are shown as means and SD values from at least three independent experiments.