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Increasing fusogenicity of selected variants during selection of the pMSCV-Clontech-X3 library. Transduced NIH-3T3 cells from each selection cycle were co-cultivated with GLVR1+ HEK-293T cells. The stringency of the selection was increased with ongoing cycles by decreasing the time available for cell-to-cell fusion. Syncytia formation was analyzed under the light-microscope at 200-fold magnification. Multinucleated cells are labeled by white lines.
