Table 1.
Clone | Selected sequenceb | Libraryc | Frequency |
---|---|---|---|
↓ | |||
WT | RISAV K I LVL R QKYQALENEGNL | control | control |
#1 | RISAGC STGPCAEIQALENEGNL- | n | 17/34 |
#7 | RISAV R K LVL D QKYQALENEGNL- | n | 1/34 |
#48 | RISAV P G LGPSPEISGSRERR- | m and n | 1/9 and 2/34 |
#12 | RISAV R R LVL P QKYQALENEGNL- | m | 1/9 |
#27 | RISAV R L LGP- | m | 1/9 |
aOnly those variants that were used for further analysis are listed.
bDiversified positions are depicted in bold, additional mutations are underlined, the R peptide cleavage site is indicated by an arrow.
cClones obtained from selection of the pMSCV-GaLV-X3-NEO library (n) or pMSCV-GaLV-X3 library (m).