Table 1.

Influence of peptide containing a tryptophan residue on T_m of dsDNA containing an AP site

DNAa	Peptideb	Tm (°C)c	ΔTm (°C)d
AP-17dsDNA	—	33.8
	11KWK	38.6	4.8
	11KAK	35.7	1.9
Native-17dsDNA	—	45.3
	11KWK	46.1	0.8
	11KAK	46.8	1.5

^aDouble-stranded oligodeoxyribonucleotides (AP-17dsDNA and Native-17dsDNA) containing an AP site or not were d-GCCAGACGHTACGACCG/d-CGGTCGTATCGTCTGGC and d-GCCAGACGATACGACCG/d-CGGTCGTATCGTCTGGC, respectively.

^bPeptides (11KWK and 11KAK) were KSRGKWKGRSA and KSRGKAKGRSK, respectively. M-dash indicate the absence of the peptide in the sample.

^cThe double-stranded oligonucleotides (17mer) were mixed with/without the peptide (11KWK or 11KAK). The mixture of the oligonucleotide (1 µM) and the peptide (0.75 µM) was dissolved in 1 mM NaCl, 10 mM sodium cacodylate (pH 7.0). Absorbance at 260 nm was measured from 20 to 70°C at the heating rate of 1°C/min.

^dΔT_m indicates the difference in which T_m (without peptide) is subtracted from T_m (with peptide).