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. 2003 Jan;77(2):1403–1414. doi: 10.1128/JVI.77.2.1403-1414.2003

FIG. 2.

FIG. 2.

The Us3 protein is found in the tegument of purified virions. (A) Diagram of the protease protection assay used in this study. Purified virions were treated with PBS (lane 1), NP-40 (lane 2), PK (lane 3), or both NP-40 and PK (lane 4). Effects of treatment on individual virus particles are shown. Note that tegument proteins remain unaffected by the first three treatments and are degraded by the fourth treatment. (B) Purified Becker, Bartha, and PRV611 (Us3 null) virions were treated with NP-40 and PK, separated on 12% polyacrylamide gels, and analyzed by Western blotting with rabbit polyclonal antiserum specific for Us3 or goat polyclonal antiserum specific for gB. The preprocessed form of gB (pgB) is labeled, as are the 69-kDa (*) and the 58-kDa (**) subunits. (C) PK15 cells were infected with PRV Bartha at an MOI of 10. At 6 h postinfection, extracts from infected and mock-infected cells were prepared and subjected to Western blot analysis with antiserum against Us3 or gB.