FIG. 9.

VP22 incorporation into Becker and Bartha virions. (A) Extracts from Becker-, Bartha-, PRV175 (VP22 null)-, and mock-infected cells were prepared at 6 h postinfection. Bartha VP22 runs more slowly in SDS-PAGE and migrates as a tight doublet, whereas Becker VP22 runs faster and migrates as a single band. The Western blot for gB served as a loading control. pgB, preprocessed form of gB. Numbers beside gels are in kilodaltons. (B) Purified Becker and Bartha virions were treated with NP-40 and PK and subjected to SDS-PAGE and Western blot analysis with VP22- or gB-specific antiserum. This film was exposed for 30 s. (C) Same blot as in panel B but with film exposed for 3 min.