TABLE 3.
The Δ30 and mutF mutations do not decrease infectivity of rDEN1 virus inoculated intrathoracically into T. splendens
| Virus | Dosea (log10 PFU) | No. tested | No. (%) infectedb |
|---|---|---|---|
| wt rDEN1 | 3.5 | 7 | 7 (100) |
| 2.5 | 8 | 6 (75) | |
| 1.5 | 7 | 5 (71) | |
| 0.5 | 5 | 3 (60) | |
| rDEN1mutF | 2.3 | 8 | 7 (88) |
| 1.3 | 5 | 4 (80) | |
| 0.3 | 8 | 7 (88) | |
| rDEN1Δ30 | 2.7 | 8 | 8 (100) |
| 1.7 | 7 | 7 (100) | |
| 0.7 | 6 | 5 (83) |
a
Virus titer administered intrathoracically in a 0.2-μl inoculum. MID50 (log10 PFU): wt rDEN1, <0.5; rDEN1mutF, <0.3; rDEN1Δ30, <0.7.
b
Number of mosquitoes with IFA-detectable antigen in head tissue prepared 21 days after inoculation.