FIG. 3.
Lipopeptides did not bind to purified HLA molecules. Purified HLA-A*0201 (A) or HLA-A*0301 (B) molecules were incubated in the presence of β2-microglobulin and different peptides or lipopeptides. They were incubated on plates previously coated with anti-HLA-A*0201 or -HLA-A*0301 monoclonal antibodies. Correctly formed HLA complexes were revealed with an anti-β2-microglobulin antibody coupled to alkaline phosphatase. Results are expressed as arbitrary fluorescence units. HLA-B27-restricted influenza virus NP383-391 and HLA-A*0201 restricted Pol476-484 were used as negative controls in A and B, respectively.