FIG. 4.
Lipopeptide presentation in the presence of monensin. HLA-A*0201 and HLA-A*0301 dendritic cells were pulsed with lipopeptides or peptides, and monensin was added 10 min later. IFN-γ production by peptide-specific CD8+ T cells was tested as in Fig. 2. Results are representative of three independent experiments. The average response in the presence of monensin compared to the uninhibited control was, for anti-Nef73-82 responses, 88% ± 15% for Nef73-82 versus 32% ± 18% for lipopeptide Nef66-97 (P < 0.05); for anti-Pol476-484 responses, 133% ± 45% for Pol476-484 versus 87% ± 6% for lipopeptide Pol461-484 (not significant).