EMSA with TD50 and increasing Pol concentrations (0, 25, 50, 100, and 200 ng). Lanes 1 to 5 contain no DBP and show DNA-Pol complexes only. The reactions in lanes 6 to 10 contain an additional 20 ng of DBP. Supershifted complexes with antibodies to Pol (lane 11) or DBP (lane 12) are indicated with an asterisk. The “+” symbol indicates the addition of DBP (20 ng), Pol (50 ng), or antibodies.