FIG. 4.

Representative plots from flow cytometry analysis of in situ-amplified WHV DNA in PBMC samples obtained from two woodchucks with residual serologically silent infection persisting after termination of acute hepatitis. PBMC obtained during acute phase of WHV hepatitis and at the indicated time points after clearance of serum WHV surface antigen and seroconversion to anti-WHV surface antigen were subjected to DNase/trypsin/DNase treatment, PCR amplification and flow cytometry analysis. The percentage of WHV DNA reactive PBMC is indicated in each panel. PBMC collected prior to inoculation with WHV from the same animals are shown as negative controls.