Figure 3. Analysis of the intracellular distribution of AGT, GO and GR/HPR in doubly transformed CHO cells by immunofluorescence microscopy.
CHO-AG cells were double-labelled for AGT (red) and GO (green) (A–C). CHO-AH cells were double-labelled either for AGT (red) and GR/HPR (green) (D–F), or MitoTracker (red) and GR/HPR (green) (G–I). CHO-GH cells were double-labelled either for MitoTracker (red) and GR/HPR (green) (J–L), or catalase (red) and GO (green) (M–O). Bar=20 μm, with all images being the same magnification. AGT and GO are mainly peroxisomal, co-localizing with catalase and with each other. GR/HPR is partly diffuse (cytosolic) and partly co-localized with MitoTracker (mitochondrial). AGT and GR/HPR do not co-localize.