Table 2. Enzyme specific activities in transformed CHO cells.
Enzyme activities are expressed as means±S.D. (n=5). Units are as follows: AGT, μmol of pyruvate utilized per h per mg of cell protein; GO, nmol of glycolate utilized per min per mg of cell protein; GR/HPR, μmol of NADPH oxidized per min per mg of cell protein. n/d, not detectable.
| Enzyme activity | |||
|---|---|---|---|
| Cell lines | AGT | GO | GR |
| CHO-O | n/d | n/d | n/d |
| CHO-A | 26.8 (±2.0) | n/d | n/d |
| CHO-G | n/d | 154.1 (±8.6) | n/d |
| CHO-H | n/d | n/d | 52.1 (±1.4) |
| CHO-AG | 22.5 (±1.4) | 122.2 (±9.7) | n/d |
| CHO-AH | 18.6 (±2.8) | n/d | 280.4 (±9.0) |
| CHO-GH | n/d | 232.1 (±4.4) | 115.6 (±6.0) |
| CHO-AGH | 2.3 (±0.2) | 110 (±14.6) | 265.1 (±43.0) |
| Reference range for normal human liver | 19.1–47.9 | 13.2–101.6 | 49–213 |
| Limit of detection | 0.24 | 0.19 | 5.5 |