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. 2006 Feb 10;394(Pt 2):449–457. doi: 10.1042/BJ20050591

Figure 2. Overexpression of Snail and Slug are associated with a full repression of Claudin-1 expression.

Figure 2

(A) Control (pcDNA3), Snail- and Slug-transfected MDCK clones were analysed by immunofluorescence and confocal microscopy for the expression of Claudin-1, E-cadherin and ZO-1. In Snail- and Slug-transfected cells, Claudin-1 and E-cadherin became almost undetectable. (B) Western blot analysis of whole-cell extracts for the indicated protein was conducted. Snail and Slug transfection caused a reduction in the protein levels of Claudin-1. (C) The reduction in the protein levels of Claudin-1 was caused by a reduction in the transcript. The presence of cClaudin-1 transcripts in control, Slug- and Snail-transfected clones was analysed by RT–PCR using different amounts of RNA. The expression of GAPDH was analysed in the same sample as a control. (D) RT–PCR for mSnail, mSlug cSnail, cSlug and GAPDH expression in control, Snail and Slug clones. (E) Expression patterns of TJ and adherens junction proteins in control and transfected cells with different levels of Snail and Slug expression. (F) RT–PCR for cClaudin-1, mSnail, mSlug and GAPDH expression in control and different Snail and Slug clones. Representative data from three independent experiments are shown.