FIG. 1.

Analysis of raft markers and detergent-resistant Gag complexes on iodixanol flotation gradients. Cells were transfected with the expression construct indicated at the right side of each panel and processed for flotation centrifugation on 50%/40%/10% iodixanol gradients in the absence of detergent (left panels) or following TX-100 extraction (right panels). The thick black bar over the top four fractions of each gradient indicates the location of membrane or DRM following centrifugation. In panels G to J, the requirements for myristylation and the I domain were examined by incorporation of myristyl-acceptor glycine substitutions in four Gag-GFP constructs. In these panels, results with both myristylated (myr+) (filled squares) and nonmyristylated (myr−) (open circles) Gag proteins are shown.