FIG. 1.
Constructs expressing SFV nsPs and polyproteins. The proteins are shown schematically on the left. Different shadings represent cleavage products (nsPs) within the nonstructural coding region. Constructs containing inactivated nsP2 protease C478ACA produce nonprocessed polyproteins, whereas wild-type polyproteins are processed to individual nsPs (see also Fig. 2C). Recombinant adeno- and baculovirus systems as well as transfection of plasmids were utilized for expression of the proteins under study.