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Purification of PBP-1. (A) The biochemical steps used in the PBP-1 purification. The final Mono-Q Sepharose column yielded the three polypeptides shown in a silver-stained 10% SDS/PAGE gel (B). (C) Fractions 21, 23, and 25 from the Mono-Q column contain the peak PBP-1 activity. (D) An immunoblot of the proteins eluted in these fractions and probed with anti-p57 antiserum.
