Figure 3.

Multiple sequence alignment of the p57 orthologs from L. seymouri (Lsp57), L. major (Lmp57, accession no. AC058781), T. brucei (Tbp57, TRYP9.0.000918; Sanger Center, Cambridge, U.K.), and HeLa SNAP50 (hSNAP50, accession no. HSU71300). The alignment was obtained by using the clustalw program. The peptides overlined above the L. seymouri sequence were obtained from peptide sequencing. The C4-type zinc finger conserved among the proteins is boxed. SNAP50 has an additional zinc finger located upstream from the boxed region. The black shade means that the residues either are identical or conserved substitution in all four sequences. The medium shade means identical or conserved substitution in three of four sequences. The light shade means the same in two sequences.