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The 57-kDa subunit of PBP-1 is a component of the PIC. (A) Transcription from purified PICs on WT (lane 1), S-Mut (lane 2), or Δ-Mut SL (lane 3) templates. (B) Protein immunoblots from each PIC, probed with a polyclonal Ab specific for the carboxyl-terminal domain of the L. seymouri RNAP II largest subunit (Upper) or a polyclonal Ab specific for the p57 subunit of PBP-1 (Lower).
