FIG. 5.

Immunoprecipitation of protease-digested products of reactions directed by mutant transcripts. (A and B) Products of reactions identical to those in Fig. 4 were digested with proteinase K and then precipitated with anti-NDV (A) or anti-Ftail (B). Precipitated material was electrophoresed on 14% polyacrylamide gels. The sizes of polypeptides indicated by arrows were determined using Rainbow markers electrophoresed on the same gel (data not shown). Fg1, Fg2, Fg3, and Fg5 are glycosylation site mutants mutated in a single-use glycosylation site. Fg1,2,3,5 encodes an F protein mutated in all four used glycosylation sites. Fd523-553 and Fd540-553 are CT deletion mutants described in Fig. 1. (C) Products of reactions directed by the wild-type transcript (Fwt) in the presence or absence of membranes (RM) and digested with protease in the presence or absence of Triton X-100. The products were then were precipitated with anti-Ftail antibody.