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. 2003 Feb;77(3):1951–1963. doi: 10.1128/JVI.77.3.1951-1963.2003

FIG. 8.

FIG. 8.

Detection of CT sequences on the surfaces of cells transfected with F-wt and F-d540-553 DNAs. (A-D) Results of flow cytometry. (A) pSVL-Fwt using anti-Ftail antibody; (B) pSVL-F-d540-553 using anti-Ftail antibody; (C) pSVL-HN using anti-Ftail antibody; (D) pSVL-Fwt (black line) and pSVL-F-d540-553 (gray line) using anti-NDV antibody. FL1-H, fluorescence. Each panel also shows cells transfected with vector alone and incubated with both primary and secondary antibody as a background control. (E to H) Detection of CT sequences on surfaces of transfected cells by immunofluorescence, (E) Vector incubated with anti-Ftail; (F) F-d540-553 incubated with anti-Ftail; (G) F-d540-553 incubated with anti-NDV; (H) F-wt incubated with anti-NDV. Panels E and F were exposed for 3 s, and panels G and H were exposed for 2 s.