Figure 5.
Effect of sst2 transfection on the death ligand-triggered signaling pathway in BxPC-3 cells. (a) Mock- and sst2-transfected BxPC-3 cells were grown (105 cells per ml) and starved for 15 h, and treated (except for control) for 4 h with TNFα + cycloheximide, TRAIL, or anti-CD95 Ab. For immunoblotting, an anti-caspase 8 antibody was used (n = 3). (b and c) Mock- and sst2-transfected BxPC-3 cells were grown (105 cells per ml) and starved for 24 h. (b) Real-time quantitative RT-PCR was performed to determine changes in the expression of TNFRI, DR4, DR5, and CD95 mRNA, respectively. Results are expressed as fold induction over mRNA expression observed in mock-transfected BxPC-3 cells and represent the mean ± SEM (n = 4). *, P < 0.05; **, P < 0.01 for sst2- vs. mock-transfected cells. (c) Immunoblots using the anti-DR4 and anti-TNFRI antibodies on whole-cell protein extracts of BxPC-3. The expression of histone was used as an internal loading control (n = 3).