FIG. 3.
Immunolocalization of VP1 (red) and β-COP (green) or VP1 (red) and Rab6 (green). 3T6 cells were infected with mouse polyomavirus (multiplicity of infection, 102 PFU per cell) and fixed at the times postinfection (p.i.) indicated (a, b, and e, 1 h postinfection; c, d, and f, 3 h postinfection). (A) Indirect immunofluorescence staining of VP1 (mouse polyclonal anti-VP1 antibody followed by Alexa Fluor-594 goat anti-mouse immunoglobulin) and COPI marker β-COP (rabbit monoclonal anti-β-COP antibody followed by Alexa Fluor-488 goat anti-rabbit immunoglobulin) was performed. (B) Staining of VP1 (mouse polyclonal anti-VP1 antibody followed by Alexa Fluor-594 goat anti-mouse immunoglobulin) and Rab6 GTPase (rabbit polyclonal anti-Rab6 antibody and Alexa Fluor-488 goat anti-rabbit immunoglobulin). Merged confocal sections are shown. Bars, 5 μm.