TABLE 5.

Influence of Vpr variants and a selected RT variant on HIV-1 mutant frequencies

RT variant	Vpr variant	Avg mutant frequency ± SD (10−1 mutants/cycle)a	Fold difference (P value)b of:
			R78A RT relative to wt RT	wt RT Vpr variants relative to wt Vpr	R78A RT Vpr variants relative to wt Vpr
wt	wt	1.61 ± 0.06
R78A	wt	0.58 ± 0.03	0.4 (<0.0005)
wt	Vpr*W54R	5.33 ± 0.06		3.3 (<0.0001)
R78A	Vpr*W54R	1.54 ± 0.03	0.3 (<0.0001)		2.7 (<0.0001)
wt	Vpr*H71R	4.71 ± 0.11		2.9 (<0.0001)
R78A	Vpr*H71R	1.16 ± 0.04	0.2 (<0.0001)		2.0 (<0.001)
wt	Vpr*H78R	4.67 ± 0.09		2.9 (<0.0001)
R78A	Vpr*H78R	1.46 ± 0.04	0.3 (<0.0001)		2.5 (<0.0001)
wt	Vpr*S79A	1.58 ± 0.05		1.0 (>0.9)
R78A	Vpr*S79A	0.53 ± 0.04	0.3 (<0.0001)		1.0 (>0.9)

^aThe average mutant frequencies ± standard deviations were determined from three independent experiments.

^bP values were determined by chi-square analysis.