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. 2002 Dec 23;100(1):183–188. doi: 10.1073/pnas.232688199

Figure 2.

Figure 2

Reduction of CCR5 surface expression on human PBLs transduced by anti-CCR5 lentiviral vector. PHA-stimulated and CD8+-depleted PBLs were transduced with various lentiviral vectors as described in Fig. 1. The transduced cells were further cultured in IL-2-containing medium for 8 days before FACS analysis for CCR5 or CXCR4 expression on the cell surface. A representative experiment with Donor B is shown. (A) Cells were stained with allophycocyanin (APC)-labeled anti-human CCR5 mAb (2D7, PharMingen). The results are exhibited as CCR5 vs. GFP dotplots with cell populations in the live lymphocyte gate (typically >75%). The quadrant lines were defined by mock-transduction and isotype-control staining, and the percentage numbers are indicated. (B) Cells were stained with APC-labeled anti-human CXCR4 mAb (CXCR4, PharMingen), and the results are plotted in the same manner as in A.