Figure 3.

Inhibition of CCR5-tropic HIV-1 infection in human PBLs transduced by lentiviral vector expressing anti-CCR5 siRNA. PBLs were transduced by various lentiviral vectors as described in Fig. 2. The transduced PBLs were cultured in IL-2-containing medium for 4 days before being challenged with either the CCR5-tropic reporter virus (A) or the CXCR4-tropic reporter virus (B). The cells were harvested 4 days after the virus challenge, and the virus-infected cells were quantitated by FACS analysis for the expression of the HSA marker. The FACS results are presented as HSA vs. GFP dotplots with cell populations in the live lymphocyte gate. The quadrant lines were defined by mock-infection and isotype-control staining, and the percentage numbers are indicated. One representative experiment with Donor B is shown.