Table 3.
Time course study on the reduction of CCR5 expression and inhibition to CCR5-tropic HIV-1 infection with the CCR5-siRNA lentivector-transduced PBLs from different donors
| Donor | Days after HIV-1 challenge | Reduction of CCR5 | HIV-1 inhibition |
|---|---|---|---|
| A | 4 | 6.99 | 3.64 |
| 7 | 6.61 | 2.64 | |
| B | 4 | 9.65 | 6.41 |
| 6 | 10.54 | 7.04 | |
| 8 | 4.88 | 5.81 | |
| C | 4 | 10.09 | 1.25 |
| 6 | 8.7 | 3.48 | |
| 8 | 9.56 | 3.73 | |
| D | 4 | 13.26 | 2.59 |
| 6 | 13.75 | 3.68 | |
| 8 | 5.68 | 4.09 |
CD8+-depleted PBLs from four different donors were transduced with various lentiviral vectors, as described in Fig. 2. Four days after the transduction, the cells were challenged with the CCR5-tropic HIV-1 reporter virus (as in Fig. 3). The virus-infected cells were analyzed by FACS for CCR5 and HSA expression at the different time points indicated. The reduction of CCR5 expression and inhibition of CCR5-tropic HIV-1 infection were determined in the same manner as in Tables 1 and 2. Equivalent degrees of CCR5 down-regulation were obtained with the similarly transduced cells but not being challenged with the HIV-1 virus.