Figure 4.

Mitotic telomeric clustering is disrupted in RNAi mutant strains, but telomeric silencing and telomere length are unaffected. (A) Deconvolved images of interphase cells subjected to immunofluorescence for Swi6 (red) and Taz1-HA (green). (B) Serial dilution analysis of wild-type and mutant strains containing the his3⁺ reporter gene inserted at the telomere of the left arm of chromosome 1. Cells were grown on nonselective medium (N/S) and medium lacking histidine (AA-HIS). (C) Telomere length is not affected in mutant strains. Genomic DNA from wild-type and mutant strains was digested with EcoRI and probed with α-³²P-labeled telomere repeat DNA (8). “MW” signifies the 1-kb molecular mass marker.