Figure 6.

Meiotic telomere clustering and the attachment of telomeres to the spindle body protein Sad1 are perturbed in the RNAi mutant strains. (A) Deconvolved images of immunofluourescence with Swi6 (red) and Taz1 (green) in meiotic cells in the horsetail stage. Class I refers to cells exhibiting the wild-type morphology with all telomeres clustered together into one spot. Class II refers to meiotic cells in which two telomere spots were clearly visible, or in which a doublet occurred such that the midpoints of the respective spots were separated by a distance greater than or equal to the longer of the two radii. Class III refers to all cells in which greater than two telomere spots were observed. (B) Deconvolved images of immunofluorescence analysis with the spindle pole body component Sad1 (red) and Taz1 (green). Representative Sad1 staining for each class is shown. (C) Frequency of classes as observed by immunofluorescence for Taz1 and Swi6 during meiosis. More than 100 meiotic cells in the horsetail stage were counted for each strain. Note different scales along the y axis.