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. 1968 Jan;14(1):21–28.

Studies on the control of antibody synthesis

Effect of antibody affinity upon its ability to supress antibody formation

J Geoffrey Walker, Gregory W Siskind
PMCID: PMC1409249  PMID: 4169723

Abstract

Suppression of anti-DNP antibody formation by passively administered anti-DNP antibody was studied quantitatively. The ability of an antiserum to suppress antibody formation was found to be related to the affinity of the antibody for the homologous antigenic determinant (ε-DNP-L-lysine), high affinity antibody being capable of causing suppression at far lower concentrations than low affinity antibody. In addition, very low concentrations of high affinity antibody were found to bring about enhancement of antibody formation. The results are discussed with respect to the significance of circulating antibody in the control of antibody synthesis.

Partial suppression of antibody formation by a single injection of passive antibody slightly lowered the affinity of the antibody synthesized. The affinity of the antibody synthesized was independent of the affinity of the passive antibody used to bring about partial suppression.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. CROWLE A. J., HU C. C. INHIBITION OF THE DEVELOPMENT OF DELAYED HYPERSENSITIVITY BY TREATMENT WITH ANTISERUM. J Immunol. 1965 Apr;94:555–562. [PubMed] [Google Scholar]
  2. EISEN H. N., SISKIND G. W. VARIATIONS IN AFFINITIES OF ANTIBODIES DURING THE IMMUNE RESPONSE. Biochemistry. 1964 Jul;3:996–1008. doi: 10.1021/bi00895a027. [DOI] [PubMed] [Google Scholar]
  3. FARAH F. S., KERN M., EISEN H. N. The preparation and some properties of purified antibody specific for the 2,4-dinitrophenyl group. J Exp Med. 1960 Dec 1;112:1195–1210. doi: 10.1084/jem.112.6.1195. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. FINKELSTEIN M. S., UHR J. W. SPECIFIC INHIBITION OF ANTIBODY FORMATION BY PASSIVELY ADMINISTERED 19S AND 7S ANTIBODY. Science. 1964 Oct 2;146(3640):67–69. doi: 10.1126/science.146.3640.67. [DOI] [PubMed] [Google Scholar]
  5. MOLLER G., WIGZELL H. ANTIBODY SYNTHESIS AT THE CELLULAR LEVEL. ANTIBODY-INDUCED SUPPRESSION OF 19S AND 7S ANTIBODY RESPONSE. J Exp Med. 1965 Jun 1;121:969–989. doi: 10.1084/jem.121.6.969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Morrison S. L., Terres G. Enhanced immunologic sensitization of mice by the simultaneous injection of antigen and specific antiserum. II. Effect of varying the antigen-antibody ratio and the amount of immune complex injected. J Immunol. 1966 May;96(5):901–905. [PubMed] [Google Scholar]
  7. OSBORN J. J., DANCIS J., JULIA J. F. Studies of the immunology of the newborn infant. II. Interference with active immunization by passive transplacental circulating antibody. Pediatrics. 1952 Sep;10(3):328–334. [PubMed] [Google Scholar]
  8. RAMACHANDRAN L. K., SASTRY L. V. Spectrometric evaluation of the approximate pK of the carboxyl group in 2,4-dinitrophenyl-amino acids. Biochemistry. 1962 Jan;1:75–78. doi: 10.1021/bi00907a012. [DOI] [PubMed] [Google Scholar]
  9. ROWLEY D. A., FITCH F. W. HOMEOSTASIS OF ANTIBODY FORMATION IN THE ADULT RAT. J Exp Med. 1964 Dec 1;120:987–1005. doi: 10.1084/jem.120.6.987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. SAHIAR K., SCHWARTZ R. S. INHIBITION OF 19S ANTIBODY SYNTHESIS BY 7S ANTIBODY. Science. 1964 Jul 24;145(3630):395–397. doi: 10.1126/science.145.3630.395. [DOI] [PubMed] [Google Scholar]
  11. SMITH R. T., EITZMAN D. V. THE DEVELOPMENT OF THE IMMUNE RESPONSE. CHARACTERIZATION OF THE RESPONSE OF THE HUMAN INFANT AND ADULT TO IMMUNIZATION WITH SALMONELLA VACCINES. Pediatrics. 1964 Feb;33:163–183. [PubMed] [Google Scholar]
  12. TERRES G., WOLINS W. Enhanced sensitization in mice by simultaneous injection of antigen and specific rabbit antiserum. Proc Soc Exp Biol Med. 1959 Oct-Dec;102:632–635. doi: 10.3181/00379727-102-25342. [DOI] [PubMed] [Google Scholar]
  13. UHR J. W., BAUMANN J. B. Antibody formation. I. The suppression of antibody formation by passively administered antibody. J Exp Med. 1961 May 1;113:935–957. doi: 10.1084/jem.113.5.935. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Velick S. F., Parker C. W., Eisen H. N. EXCITATION ENERGY TRANSFER AND THE QUANTITATIVE STUDY OF THE ANTIBODY HAPTEN REACTION. Proc Natl Acad Sci U S A. 1960 Nov;46(11):1470–1482. doi: 10.1073/pnas.46.11.1470. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Wigzell H. Antibody synthesis at the cellular level. Antibody-induced suppression of 7S antibody synthesis. J Exp Med. 1966 Nov 1;124(5):953–969. doi: 10.1084/jem.124.5.953. [DOI] [PMC free article] [PubMed] [Google Scholar]

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