FIG. 5.
IL-6 and IL-8 in tissues of LCMV-infected monkeys. Pieces of liver and spleen (100 mg) were taken from four anatomically different parts of organs and protein extracts, and RNA samples were prepared as described in Materials and Methods. (A) IL-6 protein per milligram of tissue (liver or spleen). (B) IL-6 mRNA in liver and spleen. cDNAs were amplified with IL-6 and GAPDH (glyceraldehyde-3-phosphate dehydrogenase) primers by using the GeneAmp 5700 System with SYBR Green I chemistry (34). IL-6 CT values were normalized to GAPDH amplicons, and samples from monkey Rh-ig6a were referred to as 1.0. (C) IL-8 in tissue extracts. IL-8 concentrations were measured by ELISA and expressed per milligram of tissue. Error bars indicate standard deviations.