TABLE 2.
IL-6 and TNF-α in culture media of LCMV/WE-infected cells
| Cells | Infectiona | Virus titer, 106 PFU/ml | IL-6, pg/ml (mean ± SD) | TNF-α, pg/ml (mean ± SD) |
|---|---|---|---|---|
| HepG2 | Mock | NDc | <4 | <5 |
| LPS | ND | <4 | <5 | |
| WE | 1.1 | <4 | <5 | |
| WE-UV | ND | <4 | <5 | |
| WE + LPS | 1.3 | <4 | <5 | |
| hNHepsb | Mock | ND | 414 ± 39 | <5 |
| WE | 1.2 | 397 ± 56 | <5 | |
| WE-UV | ND | 430 ± 39 | <5 | |
| WE + LPS | 1.5 | 427 ± 49 | <5 | |
| Human MDM | Mock | ND | <4 | <5 |
| LPS | ND | 7,241 ± 340 | 38,080 ± 2,365 | |
| WE | 0.1 | <4 | <5 | |
| WE-UV | ND | <4 | <5 | |
| WE + LPS | 0.08 | 7,086 ± 296 | 40,980 ± 2,963 |
a
PHA (GIBCO-BRL) and LPS from Escherichia coli 055:B55 (Sigma, St. Louis, Mo.) gave very similar results and were added to maintenance culture medium after virus adsorption to final concentrations of 5 μg/ml and 100 ng/ml, respectively. LCMV-WE was inactivated by UV light (WE-UV) as described in Materials and Methods.
b
Results with hNHep were very similar to results with human PBMC.
c
ND, not determined.