Table 1.
Experiment | CFU-MK | BFU-MK/E | BFU-E | CFU-GM | CFU-M |
---|---|---|---|---|---|
1 | 47.6 ± 6.7 | 1.3 ± 0.6 | 0.3 ± 0.6 | 0 | 0 |
2 | 49.3 ± 11.0 | 1.3 ± 1.2 | 0.3 ± 0.6 | 1.0 ± 1.0 | 0 |
3 | 46.7 ± 7.2 | 1.3 ± 1.5 | 0 | 0 | 0.3 ± 0.6 |
One hundred cells were sorted directly onto 35-mm dishes containing methylcellulose medium supplemented with SCF, Flt3-ligand, IL-3, IL-11, GM-CSF, Tpo, and Epo. Colonies were counted at day 10. Each experiment was done in triplicate. The data are presented here as the average number of colonies per dish ± SEM. BFU-MK/E, burst-forming unit–megakaryocytes/erythrocytes. BFU-E, burst-forming unit-erythrocytes.