Figure 3.

Bile acid feeding relieves the elevated hepatic CYP3A11 mRNA levels seen in the Cyp27a1^−/− mouse. Male, mixed-strain wild-type and Cyp27a1^−/− mice were fed normal chow (white bars) or chow diets containing CA (black bars) or CDCA (hatched bars) at 0.1% or 0.2% (wt/wt) for 16 days. Total RNA was isolated from individual livers, and equivalent amounts from each animal (n = 5–7) were pooled in groups for mRNA purification. Northern analyses were performed on the pooled mRNA (shown as blots in the upper part of the panel for each mRNA), quantified by PhosphorImager, and standardized against β-actin (bottom blots), and the results are shown as histograms.