Abstract
Rabbit antisera to calf collagen were investigated using columns of denatured rabbit collagen insolubilized by coupling to diazotized p-aminobenzyl cellulose. Only antibodies with general collagen specificity were bound onto the immunoadsorbent. Species specific antibodies passed through the column and were isolated in the effluent. Column-bound antibodies could be desorbed by elution with collagen peptides. The separated antibody fractions exhibited in haemagglutination—inhibition experiments with calf and rabbit collagen and collagen peptides a characteristic behaviour allowing the differentiation between them. The results support the assumption drawn from serological experiments that most antisera contain mixtures of antibodies with different specificities.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- KAMINSKI M. Study of the cross-reactions of hen and duck ovalbumins. Immunochemical relationship between native proteins and preciptating fragments obtained after proteolysis. Immunology. 1962 Mar;5:322–332. [PMC free article] [PubMed] [Google Scholar]
- RANGEL H. STUDY OF THE CROSS-REACTION BETWEEN RABBIT ANTI-BOVINE SERUM ALBUMIN ANTIBODIES AND EQUINE SERUM ALBUMIN. Immunology. 1965 Jan;8:88–94. [PMC free article] [PubMed] [Google Scholar]
- STEGEMANN H. Mikrobestimmung von Hydroxyprolin mit Chloramin-T und p-Dimethylaminobenzaldehyd. Hoppe Seylers Z Physiol Chem. 1958;311(1-3):41–45. [PubMed] [Google Scholar]
- Steffen C., Timpl R. Untersuchungen über die spezifischen Reaktionsbereiche von Kollagenantigenen. I. Nachweis der spezifischen Reaktion niedermolekularer Kollagenpeptide mit Antiseren gegen natives und denaturiertes lösliches Kollagen. Z Immunitatsforsch Allerg Klin Immunol. 1965 Dec;129(5):469–492. [PubMed] [Google Scholar]
- Timpl R., Furthmayr H., Steffen C., Doleschel W. Isolation of pure anti-collagen antibodies using a specific immunoadsorbent technique. Z Immunitatsforsch Allerg Klin Immunol. 1967 Dec;134(4):391–396. [PubMed] [Google Scholar]
- WEIGLE W. O. Immunochemical properties of the cross-reactions between anti-BSA and heterologous albumins. J Immunol. 1961 Nov;87:599–607. [PubMed] [Google Scholar]