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Effect of ubiquitination and neddylation on APP processing in 293T cells. (A) Western blot showing expression and conjugation of HisG-tagged ubiquitin and HisG-tagged Nedd-8. Lysates from transfected cells were probed with an anti-HisG antibody. (B) Effect of ubiquitin and Nedd-8 on APP processing as determined by ELISA for Aβ, β-NTF, and α-NTF.
