Skip to main content
. 2003 Feb;77(3):1812–1819. doi: 10.1128/JVI.77.3.1812-1819.2003

FIG. 3.

FIG. 3.

Mutational analysis of the PTAP and PPEY motifs of VP40. 293T cells were transfected with pCAGGS vector alone (mock, lane 1), pVP40-WT (lane 2), pVP40-P7A (lane 3), pVP40dPTA (lane 4), pVP40Y13A (lane 5), or pVP40dPT/PY (lane 6). VP40 proteins were detected in cell extracts (A) and in budding VLPs (B) by immunoprecipitation with polyclonal antiserum against the c-myc epitope tag. (C) Levels of VP40 proteins detected in VLPs were quantitated by phosphorimager analysis. The level of pVP40-WT was normalized to 100%, and the relative percentages of VP40 mutants P7A, dPTA, Y13A, and dPT/PY in VLPs are indicated.