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Recruitment of tsg101 into budding VLPs. Human 293T cells were transfected with pCAGGS (mock, lane 1), tsg101-HA alone (lane 2), or cotransfected with tsg101-HA and pVP40-WT (lane 3), pVP40-P7A (lane 4), or pVP40-dPTA (lane 5). VLPs were harvested from the culture medium at 48 h posttransfection. The presence of tsg101-HA in VLPs was detected by immunoprecipitation with anti-HA MAb. The position of tsg101-HA is indicated.
