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VP40-WT and tsg101 associate and colocalize in detergent-insoluble fractions. Vero cells were transfected with VP40-WT alone, tsg101 alone, or VP40-WT plus tsg101. Cells were lysed in 1.0% Triton X-100 at 4°C, and extracts were separated into detergent-soluble (S) and detergent-insoluble (I) fractions. VP40-WT (top panel) and tsg101 (bottom panel) were detected in soluble and/or insoluble fractions by Western blot analysis.
