Skip to main content
. 2003 Feb;77(3):1812–1819. doi: 10.1128/JVI.77.3.1812-1819.2003

FIG. 8.

FIG. 8.

A dominant-negative mutant of vps4 inhibits budding Ebola virus. Human 293T cells were transfected with vps4-WT (black bars) or vps4-E228Q (striped bars) for 24 h and then infected with Ebola virus Zaire 1976 at an MOI of 1.0. Medium samples were harvested at 3, 5, and 7 days postinfection, and titers of infectious virus were determined by plaque assays on Vero E6 cells. Cells transfected with vps4-WT yielded virus titers of 7.0 × 101, 4.5 × 104, and 6.0 × 106 PFU/ml on days 3, 5, and 7, respectively. Cells transfected with vps4-E228Q yielded virus titers of 7.5 × 100, 5.0 × 103, and 5.0 × 105 PFU/ml on days 3, 5, and 7, respectively. The virus titers shown represent the average of two independent experiments containing duplicate samples for each time point. Mock-transfected and Ebola virus-infected cells yielded virus titers of 2.0 × 107 PFU/ml on day 7 postinfection (data not shown).