Skip to main content
. 2006 Mar 17;34(5):e43. doi: 10.1093/nar/gkl011

Figure 1.

Figure 1

BENA435 is a new fluorescent DNA dye. (A) Molecular structure and the name of BENA435. (B) The 3D model of BENA435 showing a flat structure in perspective and orthogonal projections. (C) Nuclei of interphase cells stained in vivo with BENA435. Xenopus XL 177 cells were incubated in the presence of 5 µM BENA435 and illuminated using a standard Alexa488/FITC filter set. (D) Mitotic chromosomes (anaphase) stained in vivo with BENA435. (E) Absorption spectra of free and DNA-bound BENA435 at 25 µM mixed with plasmid DNA at bp/dye ratio 8. (F) Emission spectra of free and DNA-bound 1 µM BENA435. Note that upon DNA binding the peak of free BENA435 at 438 nm decreases and shifts to 426 nm, while a new peak evolves around 472–484 nm. Plasmid DNA and dA/dT homopolymer were used at bp/dye ratio 20. In both cases BENA435 was excited at 373 nm to allow the visualization of the free BENA435 fluorescence. Size bar in (C) and (D), 10 µm.