Figure 3. Activity of phage library-derived MAPs.

(a) Inhibitory activity of MAPs on PA63–LF binding in ELISA. Purified oligomeric PA63 was incubated in LF-coated wells in the presence of MAPs. (b) Inhibitory activity of MAPs on lethal-toxin-induced cell mortality. J774A.1 cells were incubated with purified PA83 and LF in the presence of MAPs. Assays were performed in triplicate and IC₅₀ values were calculated by non-linear regression analysis using GraphPad Prism 3.02 software. Results are means±S.D.