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. 2006 Mar 15;395(Pt 1):211–221. doi: 10.1042/BJ20051657

Figure 4. SDS/PAGE and Western blot analyses of chloroplastic sPPases from microalgae and plants.

Figure 4

(A) Coomassie-Blue-stained SDS/PAGE of sPPases purified from different photosynthetic organisms: cyanobacterium Pseudanabaena sp. PCC 6903, microalgae Cy. paradoxa, Cn. caldarium, Ch. reinhardtii (chloroplastic Cr-sPPase I), O. danica, Eu. gracilis and spinach chloroplasts. Molecular masses are indicated by arrows. Approx. 3 μg of purified proteins were applied per lane. (B) Western blot probed with the monospecific anti-(Cr-sPPase I) antibody showing Cr-sPPase I orthologues in diverse photosynthetic protists. Approx. 30 μg of protein extracts were loaded per lane for Chlorophyceae (Ch. reinhardtii, Ch. fusca and M. braunii) and 80 μg per lane for the other microalgae. A single protein band of 33–40 kDa was immnunodetected, except in the secondary non-photosynthetic euglenoid As. Longa, which exhibited a 60 kDa band, and the rhodophycean alga P. purpureum, where no protein was immnunodetected.