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. 2006 Mar 15;395(Pt 1):211–221. doi: 10.1042/BJ20051657

Figure 6. Cr-sPPases subcellular localization by Percoll density gradients.

Figure 6

(A) Western blots (approx. 20 μg of protein loaded per lane) with anti-(Cr-sPPase I) and anti-(Cr-sPPase II) antibodies shows that the 37 kDa Cr-sPPase I was associated with the green-coloured band (dark grey bar) and the 32 kDa Cr-sPPase II appears to be associated with a denser band, including mitochondria (light grey bar). (B) Western blot analyses of fractions 3 (cytosol) and 10 (chlorophyll peak) of the gradient with antibodies against two cytosolic protein markers in photosynthetic eukaryotes, GAPDHN (non-phosphorylating glyceraldehyde-3-phosphate dehydrogenase; left-hand panel) and GAPDHC (NAD-dependent glyceraldehyde-3-phosphate dehydrogenase; middle panel), as well as with the anti-(Cr-sPPase I) antibody (right-hand panel). Approx. 30 μg of protein was loaded per lane.