Table 2. E. coli pRGAT1 and pRGAT4 sPPase specific activities in soluble and membrane fractions.
sPPase activity (units/mg of protein) | ||||
---|---|---|---|---|
Fraction | E. coli… | pRGAT1 | pRGAT4 | pTrc99A |
Membrane | 24.0±0.12 | 11.5±0.12 | 0.8±0.04 | |
Soluble | 59.0±0.12 | 49.0±0.15 | 4.5±0.10 |