Figure 4. I-1 protein levels are changed in the skeletal muscle of glycogen synthase altered animals.
Homogenates were prepared from bulk hind-limb skeletal muscle of wild-type (WT), MGSKO (A) and GSL30 (B) mice. Extracts were separated by SDS/PAGE and proteins were transferred to membranes. Membranes were probed with an antibody to I-1 and proteins detected by chemiluminescence. The migration of the I-1 protein is shown.