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. 2006 Mar 15;395(Pt 1):203–209. doi: 10.1042/BJ20051802

Figure 2. USF activates transcription from the HP-27 gene promoter.

Figure 2

COS-7 cells were transfected with an HP-27 gene promoter-reporter plasmid, pCM27G-170/luc (white bars), or a promoterless reporter plasmid, pGV-B (black bars), together with a Renilla luciferase plasmid, pRL-SV40, which served as a control for transfection efficiency. The indicated amounts of the mammalian expression construct for mouse USF1, USF2a or USF2b were also added to the transfection mixture. The firefly luciferase activity was normalized to the Renilla luciferase activity, and the data are shown as the fold increase over the luciferase activity of pCM27G-170/luc plus pcDNA3/mUSF1 (25 ng). Results are means±S.E.M. for four separate experiments.