Figure 6. Chloride current traces, P_o^fast and P_o^common curves obtained, as before, from oocytes that co–expressed N_1–720 and C_721–988 containing the alanine substitutions E864A or E865A.

Typical sets of currents are shown for N_1–720+C_{721–988(E864A)} (A) and N_1–720+C_{721–988(E865A)} (B). Apparent P_o values for each of these E864A and E865A substitutions (C and D respectively) were derived from peak chloride currents and fitted with Boltzmann distribution curves. There is noticeably less common gating in currents from the E865A substitution and this is explained by a greater open probability of the common gates at negative potentials.