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. 2006 Mar 15;395(Pt 1):65–71. doi: 10.1042/BJ20050582

Figure 1. EGF induces ectodomain cleavage of CD44.

Figure 1

(A) EGF induces CD44 cleavage in U251MG cells in a dose-dependent manner. U251MG cells were incubated in the presence of EGF at the indicated concentrations for 2 h. To detect the CD44-cleavage product, the cells were lysed and subjected to Western blot analysis using anti-CD44cyto pAb. (B) Effects of tyrphostin A25 on EGF-induced CD44 cleavage. U251MG cells were pre-incubated in the absence (lanes 1 and 5) or presence (lanes 2 and 6, 1 μg/ml; lanes 3 and 7, 5 μg/ml; lanes 4 and 8, 20 μg/ml) of tyrphostin A25 for 1 h and were subsequently treated with 10 ng/ml EGF (lanes 5–8) or left untreated (lanes 1–4) for 2 h. The cleavage index represents the results of quantitative analysis of the CD44-cleavage product bands. Results are means±S.D. for three independent experiments.